Journal: International Journal of Molecular Sciences

Article Title: Cell-Penetrating Delivery of Nitric Oxide by Biocompatible Dinitrosyl Iron Complex and Its Dermato-Physiological Implications

doi: 10.3390/ijms221810101

Figure Lengend Snippet: Cell viability assay of the ( a ) mouse skin melanoma (B16-F10) and ( b ) human skin keratinocyte cells (HaCaT) cells, respectively, treated with different concentrations of DNIC-2 for 24 h **** p < 0.001 compared to the group without treatment of DNIC-2 . ( c ) Cell viability assay of the reconstructed human epidermis (RhE) model treated with PBS, 5% SDS, and 50 μM of DNIC-2 , respectively. ( d ) Cell viability assay of the reconstructed human cornea-like epithelium model treated with DPBS, methyl acetate, and 50 μM of DNIC-2 , respectively.

Article Snippet: CCD-966Sk human skin fibroblasts and B16-F10 mouse skin melanoma cells were purchased from Bioresource Collection and Research Center, Food Industry Research and Development Institute (Hsinchu, Taiwan), whereas human skin keratinocyte cell line, HaCaT, was purchased from Elabscience Biotechnology Inc. (Elabscience ® EP-CL-0090, Houston, TX, USA).

Techniques: Viability Assay

Journal: Arthritis Research & Therapy

Article Title: Synergistic role of c-Myc and ERK1/2 in the mitogenic response to TGFβ-1 in cultured rat nucleus pulposus cells

doi: 10.1186/ar2567

Figure Lengend Snippet: Effect of transforming growth factor β1 (TGFβ1) treatment on mRNA expression in different cell types (a), Cells were treated with or without 5 ng/mL TGFβ1 for 24 h . The expression of c-myc in nucleus pulposus cells (NP), in articular chondrocytes (AC) and keratinocytes (KT) are presented. The expression of p15 , p21 and p27 in NP was also determined. Time course of c-myc expression in NP treated with 5 ng/mL TGFβ1 (b). The graph shows the relative intensities of c-myc bands normalized for β-actin levels by densitographic analysis. Incubation for 24 h with medium containing various concentrations of fetal bovine serum (FBS) did not alter the level of c-myc expression in NP (c). The reverse transcription-polymerase chain reaction (RT-PCR) was performed on total RNA extracted from the cells. β-actin was used as an internal control.

Article Snippet: Cryopreserved primary passage rat epidermal keratinocytes were obtained from Cell Applications Inc. (San Diego, CA, USA) and maintained in growth medium (Cell Applications Inc.).

Techniques: Expressing, Incubation, Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Control

Journal: Current Research in Toxicology

Article Title: New approach methods for assessing indoor air toxicity

doi: 10.1016/j.crtox.2022.100090

Figure Lengend Snippet: The association between sampling site, reported health effects and the adverse effects detected in the biological methods.

Article Snippet: A genetically modified human keratinocyte cell line LuSens was kindly provided by BASF, Germany for research purposes.

Techniques: Sampling

Journal: Current Research in Toxicology

Article Title: New approach methods for assessing indoor air toxicity

doi: 10.1016/j.crtox.2022.100090

Figure Lengend Snippet: Description of biological and chemical analyses used to assess the quality of the indoor air samples (condensates).

Article Snippet: A genetically modified human keratinocyte cell line LuSens was kindly provided by BASF, Germany for research purposes.

Techniques: Activity Assay, Activation Assay, Purification, Binding Assay, Disruption, Concentration Assay

Journal: Current Research in Toxicology

Article Title: New approach methods for assessing indoor air toxicity

doi: 10.1016/j.crtox.2022.100090

Figure Lengend Snippet: Summary of the biological analyses of the indoor air samples. The three most sensitive assays to detect adverse effects in indoor air samples are bolded.

Article Snippet: A genetically modified human keratinocyte cell line LuSens was kindly provided by BASF, Germany for research purposes.

Techniques: Inhibition, Activity Assay, Activation Assay, Binding Assay, Disruption